The antibacterial property of silver nanoparticles has come back to the past few centuries and has fueled their reputation for medical and hygienic purposes. This study is focused on the antibacterial effects of silver nanoparticles produced by the ion-exchange method on E. coli bacteria. The bacteria growth curve was obtained so that the best time for observing the antibacterial interaction with silver nanoparticles could be determined. A cultivated bacterial suspension was then injected into a microchannel composed of an ion-exchange glass substrate that had earlier been introduced with silver ions. Statistical analysis of microscopic videos captured the bacteria's exposure to nanoparticles and silver ions revealed that as silver salt concentration increases, the characteristic time 𝜏_1/s  decreases and at the concentration of 7%,  β  declines by 40% after 10 minutes.